In vitro culture and neuron-like differentiation of human dental pulp stem cells

doi:10.3969/j.issn.2095-4344.2014.23.019

Abstract

Abstract:

BACKGROUND: The discovery and concept of pulp tissue-derived stem cells is beneficial to the understanding of tooth development and regeneration and repair mechanisms from the cellular level.

OBJECTIVE: To understand the induced differentiation capacity and induced conditions in vitro of human dental pulp stem cells into neuron-like cells.

METHODS: Pulp tissue was separated from human healthy third molars. Single cell suspensions were prepared and seeded into 6-well plates containing alpha-modified minimum essential medium supplemented with 15% fetal bovine serum. Subconfluent cultures (first passage) of colony forming cells were induced with butylhydroxy anisole, forskolin, β-mercaptoethanol, basic fibroblast growth factor.

RESULTS AND CONCLUSION: Immunofluorescence and reverse transcription-PCR assay showed that human dental pulp stem cells positively expressed stro-1, Col-I, dentin sialoprotein after 2 weeks of induction. Nestin and neuron-specific enolase were strongly expressed, but the gingival fibroblasts were negatively expressed. It indicates that adult stem cells in human dental pulp have a high neuron-like cell differentiation potential under a certain inductive condition.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, dental pulp, cell differentiation, immunophenotyping

CLC Number:

R394.2

Fang Cheng-zhi, Yang Yu-jia, Yao Yue, He Xue-hua. In vitro culture and neuron-like differentiation of human dental pulp stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(23): 3723-3726.

Figures/Tables 2

References

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